Champloo Sloppy Reborn! You have now been marked with the Brand of Sacrifice. The lives of those who bear the Brand, from the last drop of blood, to the last moment of your agonizing death, will feed life to the new Child of Darkness. The Brand of Sacrifice (生贄の焼印, Ikenie no Yakīn?) marks those anointed for the Invocation of Doom, a sacrificial ceremony in which those consecrated by the laws of causality transcend their humanity, resulting in the creation of an apostle or a God Hand member, as well as severing an apostle's last ties to humanity. The lives of the sacrificed, someone close and important to the summoner, are then offered to creatures of the darkness and ritualistically murdered. At least in the case of God Hand members, the deceased's life force is used to complete the summoner's transition. Fate Branded humans who survive these ceremonies reside in the Interstice between the physical and astral worlds, forced to fend for themselves against creatures of darkness and the nightly attacks by the restless souls of the dead who desire flesh. In response to these fell entities, the brand will bleed and cause pain. Around ordinary evil spirits or apostles, the blood will only trickle out, and the pain will be relatively minimal. Around a member of the God Hand, the brand will shoot blood out in huge gushes, and the pain inflicted will be agonizing. If the evil is too great, it can supposedly kill the Branded.